Text 650-346-8009 Tissue Culture Kits
A: Excising seeds in tissue culture. The excising kit is part of the Seed germinating kit, as a secondary "rescue" technique. Anthony can separate an embryo from its seed coat after thorough cleaning and hydrating with the kit's solutions to loosen the seed coat and minimize the damage to the embryo. The longer he can wait, the better, especially to wait until the seed has shed its coat naturally.
He may want to soak twelve seeds, plant ten in the sterile tubes to germinate normally and leave two in the hydrating vial to shed their coats. Tell him to drain the hydrating solution after removing and planting the ten tube seeds. The thin film of sterile water will be enough to allow seeds to push out their root radicle, swell and loosen their coat. Infact, the coat in the culture tube makes little difference except perhaps if it carried some small contamination.
The growth is going to come from the primary meristem as normal, and maybe from a cotyledon or part of the seed stem. Anthony will need to move up the the complete Microclone kit when the seed begins to grow in culture.
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A: We started using tissue culture tricks for exactly those reasons back in 2008 and put all of the pieces together into the Seed kits. DNA and enzymes break down over time and the risk comes from aggressive fungus attacking the energy stores before the seeds can utilize them. We use TC preparation techniques to 1) sterilize the surface of the seeds, 2) hydrate them with a sterile germination solution with essential elements and vitamins, and 3) germinating in a sterile tube of soft coco medium.
Our standard kit contains ten tubes plus two tissue culture agar tubes to rescue seeds that pause in germination, plus tools and instructions. And there are refill kits to reuse the tubes. We can also make larger custom kits and provide materials a la carte for whatever you need. As with all new skills, you should learn by using on some expendable seeds and getting the techniques and timing down.
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A: Aquatics are most often raised on multiplication media containing branching hormones, When transplanted onto Root media like ours in larger containers to develop the roots they need to anchor. We have several users who have bought aquatic plants online already in sterile culture and have split them into hundreds and even thousands of new little plants they then move onto rooting media with agar, the components in the Rootkit.
A: Yes, you would need agar to solidify. We can include the agar at no extra charge and the media would have to be resterilized in a microwave or pressure cooker if you have one. The Tissueponics media is shipped as a liquid because it is it sterile, easy to handle and pour, and does not spill or break like the agar media we used to ship in tubes.
To sterilize in a microwave, fill a one liter measuring cup with 250 ml of water and heat to boiling three times, allowing to boil about 20 seconds and noting the time. Empty the water and do the same with the Tissueponics media with agar added and dispense into tubes. Do you already have the tubes from the Tissueponics kit?
Use cloning best practices with an extra emphasis on humidity and air circulation. We use tall domes with the vents open inside of a 75% humidity chamber.
Q: Will TDZ work for all plants?
A: Thank you for asking. TDZ is more often used for woody plants but is worth trying for the plant you have. We have three different Multiplication media to try-BA, TDZ, and mT. They will all work but one may work better for you and your plant.
We make a cannabis kit and added TDZ for users wishing to copy a popular published research paper.
You may also be interested in our exclusive Tissueponics kits which skip multiplication altogether for faster results and better success. It works by rooting clones in tissue culture from small tips and nodes pruned from veg plants. See the videos on Youtube.
Q: I am wanting to propagate some houseplants, mainly philodendrons, are your media’s suitable for that...
Also once the media jars (baby food) are sterilized and filled is there a reasonable shelf life?
A: Hello, and thank you for asking. Most houseplants are tropical and prefer the yellow BA Multkit, also listed on eBay. Larger philodendrons, particularly Monstera, clone well in culture, but cutting up donor plants and getting then cleanly into culture is the challenge. You will need to carve out the growing points behind the leaves and clean them completely with a soft brush and bleach.
The media jars are good for a while if stored carefully in the same clean area the plants will be grown in. We have used media that is 6 weeks old with only a few spoiled containers from storage. If you know you are not going to use the media for a while, store the unprepared media in the fridge or freeze if sugar has already been added, until closer to the time you will need it.
Look up the cutting procedures published online and let us know when you will need media or a complete kit with tools and bottles.
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